Device

Part:BBa_K817017:Design

Designed by: Shan Chi Hsieh   Group: iGEM12_NTU-Taida   (2012-09-18)


pSB2K3 with parS site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4404
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4404
    Illegal NheI site found at 3317
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 4410
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4404
    Illegal BglII site found at 1618
    Illegal BglII site found at 2665
    Illegal XhoI site found at 3462
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4404
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4404
    Illegal XbaI site found at 4419
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1061
    Illegal NgoMIV site found at 3303
    Illegal AgeI site found at 937
    Illegal AgeI site found at 2596
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

parS site is put into biobricks cloning site not on backbone.


Source

pseudomonas putida KT2440 chromosome

References